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TitleLycopene (Chromatography)
TagsChemistry Nature Wellness Thin Layer Chromatography Carotenoid
File Size91.2 KB
Total Pages5
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                            2“Beta-carotene and other carotenoids as antioxidants.” Paiva S. A; Russell R. M. J Am Coll Nutr. 1999 Oct;18(5):424-5. < http://www.ncbi.nlm.nih.gov/pubmed/10511324> [accessed December 5 2010]
	5“The carotenoid lycopene.” Nutrition Research Newsletter. Jan, 1997. <http://findarticles.com/p/articles/mi_m0887/is_n1_v16/ai_19094812/?tag=content;col1>
                        
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DCM in hexanes, was used to elute the first color band, which was yellow (β-carotene). The

third mobile phase, DCM, was used to elute the second color band, which was scarlet (lycopene).

TLC Data: Figure 1

Fraction
Number

Rf Value β-Carotene
Rf Value

% Difference from
β-Carotene Rf Value

Identity

5 0.196 0.217 9.7% β-Carotene
6 0.327 0.327 0% β-Carotene
10 0.18 0.35 48.6% Lycopene

UV/Vis Data: Figure 2

Sampl
e

Color Sample Absorbed
wavelengths (nm)

β-Carotene Absorbed
wavelengths (nm)

Lycopene Absorbed
wavelengths (nm)

Identity

1 yello
w

426,450,476 426, 448, 474 444, 473, 502 β-Carotene

2 scarlet 444,472,502 426, 448, 474 444, 473, 502 Lycopene

Results and Discussion

The alumina gel column was very effective in separating the two carotenoids. There was

a 3 fraction gap between the last fraction containing β-carotene and the first fraction containing

lycopene. In addition, there was only one spot found for any of the fractions, which means that

each carotenoid was completely separated from the other. This is most likely the cause of using

the 3 different mobile phases, which allowed for good band separation, as well as timely elution

of the carotenoids. In addition, problems with CC encountered in previous experiment were

addressed and prevented. For example, the column never came close to running dry, which was

a problem faced in the “Synthesis and Separation of Fluorenone from Fluorene” experiment.

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